Using Cell Cultures for the Investigation of Treatments for Attention Deficit Hyperactivity Disorder: Systematic Review.
Curr Neuropharmacol. 2019 Apr 09;:
Authors: Chierrito D, Mello JCP, Villas-Boas CB, Tonin FS, Fernandez-Llimos F, Sanches ACC
BACKGROUND: Advances in basic and molecular biology have promoted the use of cell cultures in a wide range of areas, including for the evaluation of drug efficacy, safety and toxicity.
OBJECTIVE: This article was to provide a general overview of the methodological parameters of cell cultures used to investigate therapeutic options for Attention Deficit Hyperactivity Disorder.
METHOD: A systematic search was performed in the electronic databases PubMed, Scopus, and DOAJ. In vitro experimental studies using cell cultures were included.
RESULTS: A total of 328 studies were initially identified, with 16 included for qualitative synthesis. Seven studies used neuronal cells (SH-SY5Y neuroblastoma and PC12 cell line) and nine used non-neuronal cells. All studies described the culture conditions, but most studies were inconsistent with regard to reporting results and raw data. Only one-third of the studies performed cell viability assays, while a further 30% conducted gene expression analysis. Other additional tests included electrophysiological evaluation and transporter activity. More than 50% of the studies evaluated the effects of drugs such as methylphenidate and atomoxetine, while plant extracts were assessed in four studies and polyunsaturated fatty acids in one.
CONCLUSION: We suggested a flowchart to guide the planning and execution of studies, and a checklist to be completed by authors to allow the standardized reporting of results. This may guide the elaboration of laboratory protocols and further in vitro studies.
PMID: 31079591 [PubMed – as supplied by publisher]