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Novel and rapid LC-MS/MS method for quantitative analysis of methylphenidate in dried blood spots.
Bioanalysis. 2018 Jun 01;10(11):839-850
Authors: Gandhi A, Beekman C, Parker R, Fang L, Babiskin A, Matta MK
Abstract
AIM: Development and validation of a novel, sensitive, specific and rapid dried blood spots (DBS)-LC-MS/MS method for methylphenidate (MPH), an attention-deficit hyperactivity disorder drug. Methodology & results: Protein precipitation with acetonitrile was used to extract MPH from the DBS cards. Chromatographic separation was achieved on a Zorbax C18 column using an isocratic mobile phase composed of acetonitrile and 5 mM ammonium formate buffer (20:80, v/v) at a flow rate of 0.5 ml/min. MPH was quantified over a linear range of 200-25,000 pg/ml.
CONCLUSION: The clinical DBS-LC-MS/MS method was successfully validated as per the US FDA’s Bioanalytical Method Validation Guidance to support an ongoing pediatric pharmacokinetic study.
PMID: 29863895 [PubMed – indexed for MEDLINE]
via https://www.ncbi.nlm.nih.gov/pubmed/29863895?dopt=Abstract